Human PEDF (Pigment Epithelium-Derived Factor) Antibody Pair (STJAP00500)
General Information
| Short Description | This PEDF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | SERPINF1 |
| Gene ID | 5176 |
| Uniprot ID | PEDF_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human PEDF protien.Detection Immunogen: Recombinant Human PEDF protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | Neurotrophic protein.induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
| Protein Name | Pigment Epithelium-Derived FactorPedfCell Proliferation-Inducing Gene 35 ProteinEpc-1Serpin F1 |
| Database Links | |
| Cellular Localisation | SecretedMelanosomeEnriched In Stage I Melanosomes |
| Alternative Antibody Pair Names | Pigment Epithelium-Derived Factor antibody pairPedf antibody pairCell Proliferation-Inducing Gene 35 Protein antibody pairEpc-1 antibody pairSerpin F1 antibody pairSERPINF1 antibody pairPEDF antibody pairPIG35 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PEDF (Pigment Epithelium-Derived Factor) Antibody Pair (STJAP00500)
This antibody pair detects human PEDF (Pigment Epithelium-Derived Factor), corresponding to UniProt accession P36955. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated. Both are goat-derived, antigen affinity-purified, and provided at 0.5 mg/mL.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
This pair is suitable for use with serum, plasma, and other biological fluids. These are the sample types specified for this product.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Both the capture and detection antibodies were raised against recombinant human PEDF protein. Both antibodies were subsequently purified by antigen affinity purification.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.