| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human PDGFR alpha-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
0.2 Mu m filtered PBS solution, pH7.2. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The Activity was measured by the ability to bind human PDGFC-Fc in functional ELISA. NA |
| Gene Symbol: |
PDGFRA |
| Gene ID: |
5156 |
| Uniprot ID: |
PGFRA_HUMAN |
| Immunogen Region: |
ECD |
| Immunogen: |
Optimized DNA sequence encoding Human extracellular domain of human PDGF-R alpha including a C-terminal 6His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue. |
| Post Translational Modifications | N-glycosylated. Ubiquitinated, leading to its internalization and degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-731 and Tyr-742 is important for interaction with PIK3R1. Phosphorylation at Tyr-720 and Tyr-754 is important for interaction with PTPN11. Phosphorylation at Tyr-762 is important for interaction with CRK. Phosphorylation at Tyr-572 and Tyr-574 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-988 and Tyr-1018 is important for interaction with PLCG1. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor. |
| Protein Name | Platelet-Derived Growth Factor Receptor AlphaPdgf-R-AlphaPdgfr-AlphaAlpha Platelet-Derived Growth Factor ReceptorAlpha-Type Platelet-Derived Growth Factor ReceptorCd140 Antigen-Like Family Member ACd140a AntigenPlatelet-Derived Growth Factor Alpha ReceptorPlatelet-Derived Growth Factor Receptor 2Pdgfr-2Cd Antigen Cd140a |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-186763Reactome: R-HSA-186797Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6811558Reactome: R-HSA-9673767Reactome: R-HSA-9673770Reactome: R-HSA-9674396Reactome: R-HSA-9674401Reactome: R-HSA-9674403Reactome: R-HSA-9674404Reactome: R-HSA-9674428 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionCiliumGolgi Apparatus |
| Alternative Protein Names | Platelet-Derived Growth Factor Receptor Alpha proteinPdgf-R-Alpha proteinPdgfr-Alpha proteinAlpha Platelet-Derived Growth Factor Receptor proteinAlpha-Type Platelet-Derived Growth Factor Receptor proteinCd140 Antigen-Like Family Member A proteinCd140a Antigen proteinPlatelet-Derived Growth Factor Alpha Receptor proteinPlatelet-Derived Growth Factor Receptor 2 proteinPdgfr-2 proteinCd Antigen Cd140a proteinPDGFRA proteinPDGFR2 proteinRHEPDGFRA protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
