• Biotinylated Human PDGF R alpha/PDGFRA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human PDGF R alpha/PDGFRA is greater than 95% as determined by SEC-HPLC.

Human PDGF R alpha/PDGFRA protein (Recombinant) (C-His-Avi) {Biotin} (STJP001578)

SKU:
STJP001578

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human PDGF R alpha/PDGFRA-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: PDGFRA
Gene ID: 5156
Uniprot ID: PGFRA_HUMAN
Immunogen Region: Gln24-Glu524
Immunogen: DNA sequence encoding Human PDGF R alpha/PDGFRA including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications N-glycosylated. Ubiquitinated, leading to its internalization and degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-731 and Tyr-742 is important for interaction with PIK3R1. Phosphorylation at Tyr-720 and Tyr-754 is important for interaction with PTPN11. Phosphorylation at Tyr-762 is important for interaction with CRK. Phosphorylation at Tyr-572 and Tyr-574 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-988 and Tyr-1018 is important for interaction with PLCG1.
Function Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Protein Name Platelet-Derived Growth Factor Receptor Alpha
Pdgf-R-Alpha
Pdgfr-Alpha
Alpha Platelet-Derived Growth Factor Receptor
Alpha-Type Platelet-Derived Growth Factor Receptor
Cd140 Antigen-Like Family Member A
Cd140a Antigen
Platelet-Derived Growth Factor Alpha Receptor
Platelet-Derived Growth Factor Receptor 2
Pdgfr-2
Cd Antigen Cd140a
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-186763
Reactome: R-HSA-186797
Reactome: R-HSA-2219530
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9674396
Reactome: R-HSA-9674401
Reactome: R-HSA-9674403
Reactome: R-HSA-9674404
Reactome: R-HSA-9674428
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Cilium
Golgi Apparatus
Alternative Protein Names Platelet-Derived Growth Factor Receptor Alpha protein
Pdgf-R-Alpha protein
Pdgfr-Alpha protein
Alpha Platelet-Derived Growth Factor Receptor protein
Alpha-Type Platelet-Derived Growth Factor Receptor protein
Cd140 Antigen-Like Family Member A protein
Cd140a Antigen protein
Platelet-Derived Growth Factor Alpha Receptor protein
Platelet-Derived Growth Factor Receptor 2 protein
Pdgfr-2 protein
Cd Antigen Cd140a protein
PDGFRA protein
PDGFR2 protein
RHEPDGFRA protein

Information sourced from Uniprot.org

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