• TLR-2, PD-L1, GILZ and ILT3 expression on each DC condition was analyzed by flow cytometry to seek for specific tolerogenic DC markers.
  • Immunohistochemistry in macrophages/microglia 14 days postinjury (dpi) in wild-type mice for (c) PD-1, (d) it NA s ligand, PD-L1, and (e) PD-1 in M1 cells [inducible nitric oxide synthase (iNOS) +]; (f) PD-1 in M2 cells [arginase 1 (Arg1+) ]; (g) PD-L1 in M1 cells (iNOS+) ; and (h) PD-L1 in M2 cells (Arg1+).
  • Immunohistochemistry in macrophages/microglia 14 days postinjury (dpi) in wild-type mice for (c) PD-1, (d) it NA s ligand, PD-L1, and (e) PD-1 in M1 cells [inducible nitric oxide synthase (iNOS) +]; (f) PD-1 in M2 cells [arginase 1 (Arg1+) ]; (g) PD-L1 in M1 cells (iNOS+) ; and (h) PD-L1 in M2 cells (Arg1+).

Human PD-L1 (B7-H1) protein (Recombinant) (STJP000698)

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STJP000698

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human PD-L1 (B7-H1)-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by the ability of immobilized recombinant Human PD-L1 to bind recombinant human PD1 within a linear range of 1-10ug/ml in ELISA. NA
Gene Symbol: Cd274
Gene ID: 60533
Uniprot ID: PD1L1_MOUSE
Immunogen Region: PHE19-ARG238
Immunogen: Optimized DNA sequence encoding extracellular domain of Human Programmed Death Ligand 1, PD-L1, (PHE19-ARG238) including a C-terminal His tag was expressed in HEK293 cells. NA
Tissue Specificity Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin.
Post Translational Modifications Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation.
Function Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Protein Name Programmed Cell Death 1 Ligand 1
Pd-L1
Pdcd1 Ligand 1
Programmed Death Ligand 1
B7 Homolog 1
B7-H1
Cd Antigen Cd274
Database Links Reactome: R-MMU-389948
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Early Endosome Membrane
Recycling Endosome Membrane
Alternative Protein Names Programmed Cell Death 1 Ligand 1 protein
Pd-L1 protein
Pdcd1 Ligand 1 protein
Programmed Death Ligand 1 protein
B7 Homolog 1 protein
B7-H1 protein
Cd Antigen Cd274 protein
Cd274 protein
B7h1 protein
Pdcd1l1 protein
Pdcd1lg1 protein
Pdl1 protein

Information sourced from Uniprot.org

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