Host: | HEK293 |
Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Recombinant-Human PD-1/PDCD1-C-hFc-Avi protein was developed from hek293 and has a target region of C-hFc-Avi. For use in research applications. |
Conjugation: | Biotin |
Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
Dilution Range: | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
Gene Symbol: | PDCD1 |
Gene ID: | 5133 |
Uniprot ID: | PDCD1_HUMAN |
Immunogen Region: | Leu25-Gln167 |
Immunogen: | DNA sequence encoding Human PD-1/PDCD1 including a C-hFc-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
Protein Name | Programmed Cell Death Protein 1 Protein Pd-1 Hpd-1 Cd Antigen Cd279 |
Database Links | Reactome: R-HSA-389948 Reactome: R-HSA-9679191 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
Alternative Protein Names | Programmed Cell Death Protein 1 protein Protein Pd-1 protein Hpd-1 protein Cd Antigen Cd279 protein PDCD1 protein PD1 protein |
Information sourced from Uniprot.org