• Biotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.
  • The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Human PD-L1, mFc tag at 2 Mu g/ml (100 Mu l/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0. 19 Mu g/ml determined by ELISA.

Human PD-1/PDCD1 protein (Recombinant) (C-hFc-Avi) {Biotin} (STJP001670)

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STJP001670

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human PD-1/PDCD1-C-hFc-Avi protein was developed from hek293 and has a target region of C-hFc-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: PDCD1
Gene ID: 5133
Uniprot ID: PDCD1_HUMAN
Immunogen Region: Leu25-Gln167
Immunogen: DNA sequence encoding Human PD-1/PDCD1 including a C-hFc-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Function Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Protein Name Programmed Cell Death Protein 1
Protein Pd-1
Hpd-1
Cd Antigen Cd279
Database Links Reactome: R-HSA-389948
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names Programmed Cell Death Protein 1 protein
Protein Pd-1 protein
Hpd-1 protein
Cd Antigen Cd279 protein
PDCD1 protein
PD1 protein

Information sourced from Uniprot.org

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