Human PCAD (Cadherin, Placental) Sandwich ELISA Kit (STJE0019874)

SPECIFICATIONS
STJE0019874
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General Information

Short DescriptionThis PCAD Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity0.15 ng/mL
Detection Limit0.31-20 ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolCDH3
Gene ID1001
Uniprot IDCADH3_HUMAN
SpecificityThis kit recognizes Human PCAD in samples. No significant cross-reactivity or interference between Human PCAD and analogues was observed.
Sample Typeserum, plasma and other biological fluids

Additional Info

Tissue Specificity Expressed in some normal epithelial tissues and in some carcinoma cell lines.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types.
Protein Name Cadherin-3
Placental Cadherin
P-Cadherin
Database Links Reactome: R-HSA-418990
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Cadherin-3 ELISA kit
Placental Cadherin ELISA kit
P-Cadherin ELISA kit
CDH3 ELISA kit
CDHP ELISA kit
Specificity This kit recognizes Human PCAD in samples. No significant cross-reactivity or interference between Human PCAD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 102
EDTA plasma (n=8) 93-110 100
Cell culture media (n=8) 86-98 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.06 2.05 8.07 1.16 2.23 8.17
Standard deviation 0.06 0.11 0.40 0.07 0.10 0.41
CV (%) 5.74 5.33 4.95 5.90 4.67 5.06

Citations

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