| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human OSMR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
OSMR |
| Gene ID: |
9180 |
| Uniprot ID: |
OSMR_HUMAN |
| Specificity: |
This kit recognizes Human OSMR in samples. No significant cross-reactivity or interference between Human OSMR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in keratinocytes (at protein level). Expressed at relatively high levels in all neural cells as well as fibroblast and epithelial cells. |
| Function | Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events. |
| Protein Name | Oncostatin-M-Specific Receptor Subunit BetaInterleukin-31 Receptor Subunit BetaIl-31 Receptor Subunit BetaIl-31r Subunit BetaIl-31r-BetaIl-31rb |
| Database Links | Reactome: R-HSA-6788467 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Oncostatin-M-Specific Receptor Subunit Beta CLIA kitInterleukin-31 Receptor Subunit Beta CLIA kitIl-31 Receptor Subunit Beta CLIA kitIl-31r Subunit Beta CLIA kitIl-31r-Beta CLIA kitIl-31rb CLIA kitOSMR CLIA kitOSMRB CLIA kit |
| Specificity | This kit recognizes Human OSMR in samples. No significant cross-reactivity or interference between Human OSMR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-116 | 106 |
| EDTA plasma(n=8) | 85-101 | 92 |
| Cell culture media(n=8) | 93-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 104.61 | 243.26 | 725.40 | 105.97 | 257.71 | 754.98 |
| Standard deviation | 10.54 | 28.17 | 61.08 | 11.01 | 28.81 | 53.68 |
| CV (%) | 10.08 | 11.58 | 8.42 | 10.39 | 11.18 | 7.11 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
