Human NPM1 protein (Recombinant) (N-His) (STJP003761)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human NPM1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | NPM1 |
| Gene ID | 4869 |
| Uniprot ID | NPM_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gly20-Lys154 |
Additional Info
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1. |
| Protein Name | NucleophosminNpmNucleolar Phosphoprotein B23Nucleolar Protein No38Numatrin |
| Database Links | Reactome: R-HSA-180746Reactome: R-HSA-3899300Reactome: R-HSA-606279Reactome: R-HSA-6804115Reactome: R-HSA-8869496Reactome: R-HSA-9692914Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371Reactome: R-HSA-9833482 |
| Cellular Localisation | NucleusNucleolusNucleoplasmCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeGenerally NucleolarBut Is Translocated To The Nucleoplasm In Case Of Serum Starvation Or Treatment With Anticancer DrugsHas Been Found In The Cytoplasm In Patients With Primary Acute Myelogenous Leukemia (Aml)But Not With Secondary AmlCo-Localizes With The Methylated Form Of Rps10 In The Granular Component (Gc) Region Of The NucleolusColocalized With Nucleolin And Apex1 In NucleoliIsoform 1 Of Nek2 Is Required For Its Localization To The Centrosome During MitosisCan Shuttle Between Cytoplasm And Nucleus |
| Alternative Protein Names | Nucleophosmin proteinNpm proteinNucleolar Phosphoprotein B23 proteinNucleolar Protein No38 proteinNumatrin proteinNPM1 proteinNPM protein |
Information sourced from Uniprot.org