Human NOTUM protein (Recombinant-Active) (C-His) (STJP012165)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human NOTUM-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to inhibit Wnt induced TCF reporter activity in human embryonic kidney cells (HEK293). The ED50 for this effect is 0.1-0.6 Mu g/mL in the presence of 0.5 Mu g/mL of recombinant human Wnt-3a. |
Target Information
| Gene Symbol | NOTUM |
| Gene ID | 147111 |
| Uniprot ID | NOTUM_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser81-Thr451 (Cys330Ser) |
Additional Info
| Function | Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors. |
| Protein Name | Palmitoleoyl-Protein Carboxylesterase NotumHnotum |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-5362798Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Palmitoleoyl-Protein Carboxylesterase Notum proteinHnotum proteinNOTUM proteinOK proteinSW-CL.30 protein |
Information sourced from Uniprot.org