Human NKp80/KLRF1 protein (Recombinant) (hIgG-His-Tag) (STJP019495)
SPECIFICATIONS
HostHEK293 Cells
ImmunogenHuman
General Information
| Short Description | Recombinant-Human NKp80/KLRF1-hIgG-His-Tag protein was developed from hek293 cells and has a target region of hIgG-His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Bioactivity |
| Host | HEK293 Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid In Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human KLRF1 coated plates 2 ug/ml. This effect is more to 60%. |
Target Information
| Gene Symbol | KLRF1 |
| Gene ID | 51348 |
| Uniprot ID | KLRF1_HUMAN |
| Accession Number | NP_057607.1 |
| Immunogen | Human |
| Immunogen Region | 60-231aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Strongly expressed in peripheral blood leukocytes and spleen, with weaker expression in lymph node and adult liver, and no expression detected in bone marrow, thymus, and fetal liver. Not expressed in brain, heart, placenta, lung, kidney, skeletal muscle, and pancreas. Within peripheral blood leukocyte and immunocyte cell lines, expression was predominant in NK cells but was also detected in monocytes. |
| Post Translational Modifications | Phosphorylated on Tyr-7.this phosphorylation is required for NKp80/KLRF1-mediated cytotoxicity. |
| Function | Functions as an activating receptor involved in immunosurveillance upon binding to various ligands displayed at the surface of myeloid cells. Upon interaction with CLEC2B ligand, stimulates NK-cell cytotoxicity and cytokine production leading to the cytolysis of malignant CLEC2B-expressing myeloid cells. Actviation of the common cytotoxicity pathway involves SRC and SYK kinases. |
| Protein Name | Killer Cell Lectin-Like Receptor Subfamily F Member 1Lectin-Like Receptor F1Activating Coreceptor Nkp80C-Type Lectin Domain Family 5 Member C |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Protein Names | Killer Cell Lectin-Like Receptor Subfamily F Member 1 proteinLectin-Like Receptor F1 proteinActivating Coreceptor Nkp80 proteinC-Type Lectin Domain Family 5 Member C proteinKLRF1 proteinCLEC5C proteinML protein |
Information sourced from Uniprot.org