Human NFATC2 protein (Recombinant) (N-His) (STJP007606)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human NFATC2-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | NFATC2 |
| Gene ID | 4773 |
| Uniprot ID | NFAC2_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser407-Asn565 |
Additional Info
| Post Translational Modifications | In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated.which is required for full transcriptional activity. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome. |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. Is involved in the negative regulation of chondrogenesis. Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses. |
| Protein Name | Nuclear Factor Of Activated T-Cells - Cytoplasmic 2Nf-Atc2Nfatc2Nfat Pre-Existing SubunitNf-AtpT-Cell Transcription Factor Nfat1 |
| Database Links | Reactome: R-HSA-2025928Reactome: R-HSA-2871809Reactome: R-HSA-5607763Reactome: R-HSA-8877330 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated DephosphorylationRapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium SignalsThe Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription |
| Alternative Protein Names | Nuclear Factor Of Activated T-Cells - Cytoplasmic 2 proteinNf-Atc2 proteinNfatc2 proteinNfat Pre-Existing Subunit proteinNf-Atp proteinT-Cell Transcription Factor Nfat1 proteinNFATC2 proteinNFAT1 proteinNFATP protein |
Information sourced from Uniprot.org