| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human NELFE-N-His-SUMO & C-Strep protein was developed from e. coli and has a target region of N-His-SUMO & C-Strep. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | NELFE |
| Gene ID: | 7936 |
| Uniprot ID: | NELFE_HUMAN |
| Immunogen Region: | Gly261-Arg331 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin. Sumoylated. Poly-ADP-ribosylated by PARP1, thereby preventing RNA-binding and relieving transcription pausing. |
| Function | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. |
| Protein Name | Negative Elongation Factor ENelf-ERna-Binding Protein Rd |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeLocalizes To ChromatinPhosphorylation By The P-Tefb Complex Promotes Its Release From Chromatin |
| Alternative Protein Names | Negative Elongation Factor E proteinNelf-E proteinRna-Binding Protein Rd proteinNELFE proteinRD proteinRDBP protein |
Information sourced from Uniprot.org