Human NEFL (Neurofilament Light Polypeptide) Antibody Pair (STJAP00455)
General Information
| Short Description | This NEFL antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | NEFL |
| Gene ID | 4747 |
| Uniprot ID | NFL_HUMAN |
| Immunogen | Capture immunogen: Natural Human NEFL protien.Detection Immunogen: Natural Human NEFL protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Light PolypeptideNf-L68 Kda Neurofilament ProteinNeurofilament Triplet L Protein |
| Database Links | Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-5673001Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell ProjectionAxonCytoplasmCytoskeleton |
| Alternative Antibody Pair Names | Neurofilament Light Polypeptide antibody pairNf-L antibody pair68 Kda Neurofilament Protein antibody pairNeurofilament Triplet L Protein antibody pairNEFL antibody pairNF68 antibody pairNFL antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human NEFL (Neurofilament Light Polypeptide) Antibody Pair (STJAP00455)
This antibody pair detects Human Neurofilament Light Polypeptide (NEFL, UniProt P07196) and has confirmed reactivity with human samples only.
The capture antibody is unconjugated and the detection antibody is biotinylated, with both supplied at 0.5 mg/mL. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This antibody pair is validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Suitable sample types include serum, plasma, and other biological fluids of human origin.
Both the capture and detection antibodies were raised against natural human NEFL protein. The capture antibody was generated in rat and the detection antibody in rabbit, with both purified by Protein A or G affinity chromatography.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to preserve antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.