| Host: |
E. coli |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human NEDD8-C-His protein was developed from e. coli and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of 20mM TRIS, 150mM NaCl, pH 8.0. Contact us for customized product form or formulation. |
| Endotoxin: |
Please contact us for more information. |
| Gene Symbol: |
NEDD8 |
| Gene ID: |
4738 |
| Uniprot ID: |
NEDD8_HUMAN |
| Immunogen Region: |
Met1-Gly76 |
| Immunogen: |
Recombinant Human NEDD8 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Gly76) of human NEDD8 (Accession #NP_006147.1) fused with a 6×His tag at the C-terminus. |
| Tissue Specificity | Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. |
| Post Translational Modifications | Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. (Microbial infection) Deamidated at Gln-40 by bacterial cyclomodulin Cif produced by enteropathogenic E.coli, Y.pseudotuberculosis or B.pseudomallei, leading to impair NEDD8 ability to activate cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes). Deamidation occurs on NEDD8-modified cullins. NEDD8 deamidation prevents switching from the inactive to active state by maintaining the 'closed' structure of the CRL complexes. Deamidation may also impair its deconjugation by the COP9 signalosome.However this result needs additional evidences. |
| Function | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. |
| Protein Name | Nedd8NeddylinNeural Precursor Cell Expressed Developmentally Down-Regulated Protein 8Nedd-8Ubiquitin-Like Protein Nedd8 |
| Database Links | Reactome: R-HSA-2173789Reactome: R-HSA-5689603Reactome: R-HSA-8856825Reactome: R-HSA-8951664Reactome: R-HSA-917937 |
| Cellular Localisation | NucleusMainly Nuclear |
| Alternative Protein Names | Nedd8 proteinNeddylin proteinNeural Precursor Cell Expressed Developmentally Down-Regulated Protein 8 proteinNedd-8 proteinUbiquitin-Like Protein Nedd8 proteinNEDD8 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
