Human NCF1 (Neutrophil Cytosolic Factor 1) Sandwich ELISA Kit (STJE0010891)
SPECIFICATIONS
General Information
| Short Description | This NCF1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.117ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | NCF1 |
| Gene ID | 653361 |
| Uniprot ID | NCF1_HUMAN |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Detected in peripheral blood monocytes and neutrophils (at protein level). |
| Post Translational Modifications | Phosphorylated by PRKCD.phosphorylation induces activation of NCF1, leading to assembly and activation of the NADPH oxidase complex. |
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. |
| Protein Name | Neutrophil Cytosol Factor 1Ncf-147 Kda Autosomal Chronic Granulomatous Disease Protein47 Kda Neutrophil Oxidase FactorNcf-47kNeutrophil Nadph Oxidase Factor 1Nox Organizer 2Nox-Organizing Protein 2Sh3 And Px Domain-Containing Protein 1aP47-Phox |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423 |
| Cellular Localisation | CytoplasmCytosolMembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative ELISA Names | Neutrophil Cytosol Factor 1 ELISA kitNcf-1 ELISA kit47 Kda Autosomal Chronic Granulomatous Disease Protein ELISA kit47 Kda Neutrophil Oxidase Factor ELISA kitNcf-47k ELISA kitNeutrophil Nadph Oxidase Factor 1 ELISA kitNox Organizer 2 ELISA kitNox-Organizing Protein 2 ELISA kitSh3 And Px Domain-Containing Protein 1a ELISA kitP47-Phox ELISA kitNCF1 ELISA kitNOXO2 ELISA kitSH3PXD1A ELISA kit |
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Information sourced from Uniprot.org