Human MUC-16/CA125 protein (Recombinant) (C-His&Avi) {Biotin} (STJP020073)
SPECIFICATIONS
HostHEK293 cells
ConjugationBiotin
ImmunogenBiotinylated Recombinant Human MUC-16/CA125 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gly12660-Met12923) of Human MUC-16/CA125 fused with a His and Avi tag at the C-terminal.
General Information
| Short Description | Recombinant-Human MUC-16/CA125-C-His&Avi protein was developed in hek293 cells using the region Gly12660-Met12923. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MUC16 |
| Gene ID | 94025 |
| Uniprot ID | MUC16_HUMAN |
| Immunogen | Biotinylated Recombinant Human MUC-16/CA125 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gly12660-Met12923) of Human MUC-16/CA125 fused with a His and Avi tag at the C-terminal. |
| Immunogen Region | Gly12660-Met12923 |
Additional Info
| Tissue Specificity | Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. |
| Post Translational Modifications | Heavily O-glycosylated.expresses both type 1 and type 2 core glycans. Heavily N-glycosylated.expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. |
| Function | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. |
| Protein Name | Mucin-16Muc-16Ovarian Cancer-Related Tumor Marker Ca125Ca-125Ovarian Carcinoma Antigen Ca125 |
| Database Links | Reactome: R-HSA-5083625Reactome: R-HSA-5083632Reactome: R-HSA-5083636Reactome: R-HSA-5621480Reactome: R-HSA-913709Reactome: R-HSA-977068 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSecretedExtracellular SpaceMay Be Liberated Into The Extracellular Space Following The Phosphorylation Of The Intracellular C-Terminus Which Induces The Proteolytic Cleavage And Liberation Of The Extracellular Domain |
| Alternative Protein Names | Mucin-16 proteinMuc-16 proteinOvarian Cancer-Related Tumor Marker Ca125 proteinCa-125 proteinOvarian Carcinoma Antigen Ca125 proteinMUC16 proteinCA125 protein |
Information sourced from Uniprot.org