| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of 0.1% TFA, 30% ACN. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 24.59-98.36 ng/mL, corresponding to a specific activity of 1.02×104~4.07×104 units/mg. |
| Gene Symbol: | BMP2 |
| Gene ID: | 650 |
| Uniprot ID: | BMP2_HUMAN |
| Immunogen Region: | Ala284-Arg396 |
| Immunogen: | Recombinant Human/Mouse/Rat mature BMP-2 Protein is produced by Escherichia coli expression system. The target protein is expressed with sequence (Ala284-Arg396) of Human/Mouse/Rat BMP-2 (NP_001191.1) fused with no tag. |
| Immunogen Sequence: | LKTLHLGSCVITANLQAIQK EFSEIRDSVQAEDTNIDIRI LRTTESLKDIKSLDRCCFLR HLVRFYLDRVFKVYQTPDHH TLRKISSLANSFLIIKKDLS VCHSHMACHCGEEAMEKYNQ ILSHFIELELQAAVVKALGE LGILLRWMEEML |
| Tissue Specificity | Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. |
| Function | Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Induces cartilage and bone formation. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation. Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling. |
| Protein Name | Bone Morphogenetic Protein 2 Bmp-2 Bone Morphogenetic Protein 2a Bmp-2a |
| Database Links | Reactome: R-HSA-201451 Reactome: R-HSA-2129379 Reactome: R-HSA-8878166 Reactome: R-HSA-8939902 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Bone Morphogenetic Protein 2 protein Bmp-2 protein Bone Morphogenetic Protein 2a protein Bmp-2a protein BMP2 protein BMP2A protein |
Information sourced from Uniprot.org