| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to induce p38 MAPK activation in 3T3 L1 mouse embryonic fibroblast adipose-like cells. 0.1 Mu g/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation. |
| Gene Symbol: | FNDC5 |
| Gene ID: | 252995 |
| Uniprot ID: | FNDC5_HUMAN |
| Immunogen Region: | Asp32-Glu143 |
| Immunogen: | Recombinant Human/Mouse/Rat Irisin/FNDC5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp32-Glu143) of Human/Mouse/Rat Irisin (Accession #NP_715637.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | DSPSAPVNVTVRHLKANSAV VSWDVLEDEVVIGFAISQQK KDVRMLRFIQEVNTTTRSCA LWDLEEDTEYIVHVQAISIQ GQSPASEPVLFKTPREAEKM ASKNKDEVTMKE |
| Post Translational Modifications | The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated. |
| Function | Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. |
| Protein Name | Fibronectin Type Iii Domain-Containing Protein 5Fibronectin Type Iii Repeat-Containing Protein 2 Cleaved Into - Irisin |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPeroxisome MembraneImported In Peroxisomes Through The Pex5 Receptor PathwayIrisin: SecretedDetected In The Blood Of Individuals Subjected To Endurance Exercise |
| Alternative Protein Names | Fibronectin Type Iii Domain-Containing Protein 5 proteinFibronectin Type Iii Repeat-Containing Protein 2 Cleaved Into - Irisin proteinFNDC5 proteinFRCP2 protein |
Information sourced from Uniprot.org