Human MMP1 (Matrix Metalloproteinase 1) Sandwich ELISA Kit (STJE0004085)

Name: Human MMP1 (Matrix Metalloproteinase 1) Sandwich ELISA Kit (STJE0004085)
SKU: STJE0004085
Availability: InStock

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SPECIFICATIONS

STJE0004085

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Short Description	This MMP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	MMP1
Gene ID	4312
Uniprot ID	MMP1_HUMAN
Specificity	This kit recognizes Human MMP-1 in samples. No significant cross-reactivity or interference between Human MMP-1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Protein Name	Interstitial Collagenase Fibroblast Collagenase Matrix Metalloproteinase-1 Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1474228 Reactome: R-HSA-1592389 Reactome: R-HSA-210991 Reactome: R-HSA-381426 Reactome: R-HSA-6785807
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative ELISA Names	Interstitial Collagenase ELISA kit Fibroblast Collagenase ELISA kit Matrix Metalloproteinase-1 ELISA kit Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase ELISA kit MMP1 ELISA kit CLG ELISA kit
Specificity	This kit recognizes Human MMP-1 in samples. No significant cross-reactivity or interference between Human MMP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	85.33	246.42	880.11	82.77	242.81	921.97
Standard deviation	4.95	11.58	36.08	4.59	11.07	47.02
CV (%)	5.80	4.70	4.10	5.55	4.56	5.10