Human Midkine (Midkine) Antibody Pair (STJAP00894)
General Information
| Short Description | This Midkine antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R02-9I-6/R07-6N-9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MDK |
| Gene ID | 4192 |
| Uniprot ID | MK_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions. |
| Function | Secreted protein that functions as a cytokine and growth factor and mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptors via their chondroitin sulfate (CS) groups. Thereby regulates many processes like inflammatory response, cell proliferation, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation and cell migration. Participates in inflammatory processes by exerting two different activities. Firstly, mediates neutrophils and macrophages recruitment to the sites of inflammation both by direct action by cooperating namely with ITGB2 via LRP1 and by inducing chemokine expression. This inflammation can be accompanied by epithelial cell survival and smooth muscle cell migration after renal and vessel damage, respectively. Secondly, suppresses the development of tolerogenic dendric cells thereby inhibiting the differentiation of regulatory T cells and also promote T cell expansion through NFAT signaling and Th1 cell differentiation. Promotes tissue regeneration after injury or trauma. After heart damage negatively regulates the recruitment of inflammatory cells and mediates cell survival through activation of anti-apoptotic signaling pathways via MAPKs and AKT pathways through the activation of angiogenesis. Also facilitates liver regeneration as well as bone repair by recruiting macrophage at trauma site and by promoting cartilage development by facilitating chondrocyte differentiation. Plays a role in brain by promoting neural precursor cells survival and growth through interaction with heparan sulfate proteoglycans. Binds PTPRZ1 and promotes neuronal migration and embryonic neurons survival. Binds SDC3 or GPC2 and mediates neurite outgrowth and cell adhesion. Binds chondroitin sulfate E and heparin leading to inhibition of neuronal cell adhesion induced by binding with GPC2. Binds CSPG5 and promotes elongation of oligodendroglial precursor-like cells. Also binds ITGA6:ITGB1 complex.this interaction mediates MDK-induced neurite outgrowth. Binds LRP1.promotes neuronal survival. Binds ITGA4:ITGB1 complex.this interaction mediates MDK-induced osteoblast cells migration through PXN phosphorylation. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Promotes epithelial to mesenchymal transition through interaction with NOTCH2. During arteriogenesis, plays a role in vascular endothelial cell proliferation by inducing VEGFA expression and release which in turn induces nitric oxide synthase expression. Moreover activates vasodilation through nitric oxide synthase activation. Negatively regulates bone formation in response to mechanical load by inhibiting Wnt/beta-catenin signaling in osteoblasts. In addition plays a role in hippocampal development, working memory, auditory response, early fetal adrenal gland development and the female reproductive system. |
| Protein Name | MidkineMkAmphiregulin-Associated ProteinArapMidgestation And Kidney ProteinNeurite Outgrowth-Promoting Factor 2Neurite Outgrowth-Promoting Protein |
| Database Links | Reactome: R-HSA-201556Reactome: R-HSA-2979096Reactome: R-HSA-9851151 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Midkine antibody pairMk antibody pairAmphiregulin-Associated Protein antibody pairArap antibody pairMidgestation And Kidney Protein antibody pairNeurite Outgrowth-Promoting Factor 2 antibody pairNeurite Outgrowth-Promoting Protein antibody pairMDK antibody pairMK1 antibody pairNEGF2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Midkine (Midkine) Antibody Pair (STJAP00894)
This antibody pair detects human Midkine (UniProt P21741) and has confirmed reactivity with human samples only. Researchers working with non-human species should validate cross-reactivity independently before use.
Both antibodies in this pair are rabbit-derived monoclonal IgGs, with clone IDs R02-9I-6 and R07-6N-9. Both were raised against a recombinant protein immunogen and purified by affinity chromatography.
This antibody pair is validated for use in ELISA. It is intended for sandwich ELISA development, though the working dilution for each antibody must be determined by the end user for their specific experimental conditions.
Both antibodies are supplied unconjugated and formulated in 0.01M PBS at pH 7.2. Researchers developing a sandwich ELISA will need to conjugate the detection antibody to an appropriate reporter, such as biotin or HRP, using standard labeling methods.
The antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Concentration and purity data are not specified in the current product details for this pair. Researchers requiring this information for assay development should contact St John's Laboratory directly to request a certificate of analysis.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.