| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Loaded Human Mesothelin/MSLN Protein, C-hFc&His on ProA Biosensor, can bind Human CA125 Protein, C-His&Avi with an affinity constant of 17.2 nM as determined in BLI assay (Gator). |
| Gene Symbol: | MSLN |
| Gene ID: | 10232 |
| Uniprot ID: | MSLN_HUMAN |
| Immunogen: | Recombinant Human Mesothelin/MSLN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu296-Gly580) of human Mesothelin (Accession #NP_005814.2) fused with a Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: | EVEKTACPSGKKAREIDESL IFYKKWELEACVDAALLATQ MDRVNAIPFTYEQLDVLKHK LDELYPQGYPESVIQHLGYL FLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQVATLI DRFVKGRGQLDKDTLDTLTA FYPGYLCSLSPEELSSVPPS SIWAVRPQDLDTCDPRQLDV LYPKARLAFQNMNGSEYFVK IQSFLGGAPTEDLKALSQQN VSMDLATFMKLRTDAVLPL |
| Post Translational Modifications | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. |
| Protein Name | MesothelinCak1 AntigenPre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating FactorMpf - Mesothelin - Cleaved Form |
| Database Links | Reactome: R-HSA-163125Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorGolgi ApparatusMegakaryocyte-Potentiating Factor: SecretedIsoform 3: Secreted |
| Alternative Protein Names | Mesothelin proteinCak1 Antigen proteinPre-Pro-Megakaryocyte-Potentiating Factor Cleaved Into - Megakaryocyte-Potentiating Factor proteinMpf - Mesothelin - Cleaved Form proteinMSLN proteinMPF protein |
Information sourced from Uniprot.org