Human MBD1 protein (Recombinant) (N-His) (STJP006173)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human MBD1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | MBD1 |
| Gene ID | 4152 |
| Uniprot ID | MBD1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Leu216 |
Additional Info
| Post Translational Modifications | Sumoylated, sumoylation may increase interaction with ATF7IP. |
| Function | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. |
| Protein Name | Methyl-Cpg-Binding Domain Protein 1Cxxc-Type Zinc Finger Protein 3Methyl-Cpg-Binding Protein Mbd1Protein Containing Methyl-Cpg-Binding Domain 1 |
| Database Links | Reactome: R-HSA-3899300 |
| Cellular Localisation | NucleusNucleus MatrixNucleus SpeckleChromosomeNuclearIn A Punctate PatternAssociated With Euchromatic Regions Of The ChromosomesWith Pericentromeric Regions On Chromosome 1 And With Telomeric Regions From Several Chromosomes |
| Alternative Protein Names | Methyl-Cpg-Binding Domain Protein 1 proteinCxxc-Type Zinc Finger Protein 3 proteinMethyl-Cpg-Binding Protein Mbd1 proteinProtein Containing Methyl-Cpg-Binding Domain 1 proteinMBD1 proteinCXXC3 proteinPCM1 protein |
Information sourced from Uniprot.org