Human MAPK8 protein (Recombinant) (N-His) (STJP004760)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human MAPK8-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | MAPK8 |
| Gene ID | 5599 |
| Uniprot ID | MK08_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Tyr26-Ile321 |
Additional Info
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses. Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly. Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity. Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome. Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation. JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. |
| Protein Name | Mitogen-Activated Protein Kinase 8Map Kinase 8Mapk 8Jnk-46Stress-Activated Protein Kinase 1cSapk1cStress-Activated Protein Kinase Jnk1C-Jun N-Terminal Kinase 1 |
| Database Links | Reactome: R-HSA-111446Reactome: R-HSA-139910Reactome: R-HSA-193648Reactome: R-HSA-205043Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-450341Reactome: R-HSA-5693565Reactome: R-HSA-9007892Reactome: R-HSA-9673324Reactome: R-HSA-9725370 |
| Cellular Localisation | CytoplasmNucleusSynapseIn The Cortical NeuronsPredominantly Cytoplasmic And Associated With The Golgi Apparatus And Endosomal FractionIncreased Neuronal Activity Increases Phosphorylated Form At SynapsesColocalizes With Pou5f1 In The Nucleus |
| Alternative Protein Names | Mitogen-Activated Protein Kinase 8 proteinMap Kinase 8 proteinMapk 8 proteinJnk-46 proteinStress-Activated Protein Kinase 1c proteinSapk1c proteinStress-Activated Protein Kinase Jnk1 proteinC-Jun N-Terminal Kinase 1 proteinMAPK8 proteinJNK1 proteinPRKM8 proteinSAPK1 proteinSAPK1C protein |
Information sourced from Uniprot.org