| Host: | B.mori |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human M-CSF-protein was developed from b.mori. For use in research applications. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution pH7.0 containing % HSA, % mannitol. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | Activity was tested by binding recombinant Human MCSF receptor in a functional ELISA assay with a linear range of 1-5 ng/ml. NA |
| Gene Symbol: | CSF1 |
| Gene ID: | 1435 |
| Uniprot ID: | CSF1_HUMAN |
| Immunogen Region: | Mature chain |
| Immunogen: | DNA sequence encoding Human M-CSF mature chain fused to polyHis AVI tag was expressed in baculovirus infected Bombyx Mori. NA |
| Immunogen Sequence: | MTAPGAAGRC PPTTWLGSLL LLVCLLASRS ITEEVSEYCS HMIGSGHLQS LQRLIDSQME TSCQITFEFV DQEQLKDPVC YLKKAFLLVQ DIMEDTMRFR DNTPNAIAIV QLQELSLRLK SCFTKDYEEH DKACVRTFYE TPLQLLEKVK NVFNETKNLL DKDWNIFSKN CNNSFAECSS Q DVVTKPDCN CLYPKAIPSS DPASVSPHQP LAPSMAPVAG LTWEDSE |
| Post Translational Modifications | N-glycosylated. Isoform 1: N-glycosylated. Isoform 3: N-glycosylated. O-glycosylated.contains chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1: O-glycosylated. |
| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. |
| Protein Name | Macrophage Colony-Stimulating Factor 1 Csf-1 M-Csf Mcsf Lanimostim Proteoglycan Macrophage Colony-Stimulating Factor Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit |
| Database Links | Reactome: R-HSA-381426 Reactome: R-HSA-449836 Reactome: R-HSA-6783783 Reactome: R-HSA-8957275 Reactome: R-HSA-9680350 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Processed Macrophage Colony-Stimulating Factor 1: Secreted Extracellular Space |
| Alternative Protein Names | Macrophage Colony-Stimulating Factor 1 protein Csf-1 protein M-Csf protein Mcsf protein Lanimostim protein Proteoglycan Macrophage Colony-Stimulating Factor protein Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit protein CSF1 protein |
Information sourced from Uniprot.org