| Host: |
Mouse/Mouse |
| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This LpPLA2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Clonality: |
Monoclonal/Monoclonal |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Purification: |
Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: |
PLA2G7 |
| Gene ID: |
7941 |
| Uniprot ID: |
PAFA_HUMAN |
| Immunogen: |
Capture immunogen: Recombinant Human LpPLA2 protein.Detection Immunogen: Recombinant Human LpPLA2 protein. |
| Post Translational Modifications | N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles. |
| Function | Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage. |
| Protein Name | Platelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase1-Alkyl-2-Acetylglycerophosphocholine Esterase2-Acetyl-1-Alkylglycerophosphocholine EsteraseGroup-Viia Phospholipase A2Gviia-Pla2Ldl-Associated Phospholipase A2Ldl-Pla(2Paf 2-Acylhydrolase |
| Database Links | Reactome: R-HSA-422085 |
| Cellular Localisation | SecretedExtracellular SpaceAssociates With Both Ldl And Hdl Particles In PlasmaMainly Associates With Pro-Inflammatory Electronegative Ldl Particles |
| Alternative Antibody Pair Names | Platelet-Activating Factor Acetylhydrolase antibody pairPaf Acetylhydrolase antibody pair1-Alkyl-2-Acetylglycerophosphocholine Esterase antibody pair2-Acetyl-1-Alkylglycerophosphocholine Esterase antibody pairGroup-Viia Phospholipase A2 antibody pairGviia-Pla2 antibody pairLdl-Associated Phospholipase A2 antibody pairLdl-Pla(2 antibody pairPaf 2-Acylhydrolase antibody pairPLA2G7 antibody pairPAFAH antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
