Human LIPE protein (Recombinant) (N-His) (STJP005188)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP005188
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General Information

Short DescriptionRecombinant-Human LIPE-N-His protein was developed from e.coli for the region N-His. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolLIPE
Gene ID3991
Uniprot IDLIPS_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionMet302-Leu425

Additional Info

Post Translational Modifications Phosphorylation by AMPK reduces its translocation towards the lipid droplets.
Function Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Protein Name Hormone-Sensitive Lipase
Hsl
Monoacylglycerol Lipase Lipe
Retinyl Ester Hydrolase
Reh
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-9841922
Cellular Localisation Cell Membrane
Membrane
Caveola
Cytoplasm
Cytosol
Lipid Droplet
Found In The High-Density Caveolae
Translocates To The Cytoplasm From The Caveolae Upon Insulin Stimulation
Phosphorylation By Ampk Reduces Its Translocation Towards The Lipid Droplets
Alternative Protein Names Hormone-Sensitive Lipase protein
Hsl protein
Monoacylglycerol Lipase Lipe protein
Retinyl Ester Hydrolase protein
Reh protein
LIPE protein

Information sourced from Uniprot.org

Citations

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