| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human LILRB2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
LILRB2 |
| Gene ID: |
10288 |
| Uniprot ID: |
LIRB2_HUMAN |
| Specificity: |
This kit recognizes Human LILRB2 in samples. No significant cross-reactivity or interference between Human LILRB2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells. Expressed in myeloid-derived suppressor cells during pregnancy. Detected at low levels in natural killer (NK) cells. Expressed in B cells. |
| Post Translational Modifications | Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. |
| Function | Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. |
| Protein Name | Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2Lir-2Leukocyte Immunoglobulin-Like Receptor 2Cd85 Antigen-Like Family Member DImmunoglobulin-Like Transcript 4Ilt-4Monocyte/Macrophage Immunoglobulin-Like Receptor 10Mir-10Cd Antigen Cd85d |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 CLIA kitLir-2 CLIA kitLeukocyte Immunoglobulin-Like Receptor 2 CLIA kitCd85 Antigen-Like Family Member D CLIA kitImmunoglobulin-Like Transcript 4 CLIA kitIlt-4 CLIA kitMonocyte/Macrophage Immunoglobulin-Like Receptor 10 CLIA kitMir-10 CLIA kitCd Antigen Cd85d CLIA kitLILRB2 CLIA kitILT4 CLIA kitLIR2 CLIA kitMIR10 CLIA kit |
| Specificity | This kit recognizes Human LILRB2 in samples. No significant cross-reactivity or interference between Human LILRB2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 92 |
| EDTA plasma(n=8) | 101-117 | 108 |
| Cell culture media(n=8) | 91-106 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 93.76 | 263.95 | 925.19 | 87.48 | 257.17 | 1005.66 |
| Standard deviation | 7.70 | 19.56 | 102.88 | 8.91 | 26.31 | 115.35 |
| CV (%) | 8.21 | 7.41 | 11.12 | 10.19 | 10.23 | 11.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
