| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng/mL, corresponding to a specific activity of 3.33×106-1×107units/mg. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | LIF |
| Gene ID: | 3976 |
| Uniprot ID: | LIF_HUMAN |
| Immunogen: | Recombinant Human Leukemia inhibitory factor/LIF Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser23-Phe202) of human LIF (Accession #NP_002300.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region: | Ser23-Phe202 |
| Immunogen Sequence: | SPLPITPVNATCAIRHPCHN NLMNQIRSQLAQLNGSANAL FILYYTAQGEPFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYLGTSLGNITRD QKILNPSALSLHSKLNATAD ILRGLLSNVLCRLCSKYHVG HVDVTYGPDTSGKDVFQKKK LGCQLLGKYKQIIAVLAQAF |
| Protein Name | Leukemia Inhibitory FactorLifDifferentiation-Stimulating FactorD FactorMelanoma-Derived Lpl InhibitorMlpliEmfilermin |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-6788467 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Leukemia Inhibitory Factor proteinLif proteinDifferentiation-Stimulating Factor proteinD Factor proteinMelanoma-Derived Lpl Inhibitor proteinMlpli proteinEmfilermin proteinLIF proteinHILDA protein |
Information sourced from Uniprot.org
