| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human LDL receptor-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Biotin |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was tested by the ability of immobilized recombinant Human PCSK9 to bind biotinylated recombinant human LDL Receptor in ELISA. The ED50 was calculated to be 0.6ug/ml. NA |
| Gene Symbol: |
LDLR |
| Gene ID: |
3949 |
| Uniprot ID: |
LDLR_HUMAN |
| Immunogen Region: |
ALA22-ARG788 |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of Human Low density lipoprotein receptor (ALA22-ARG788) including a C-terminal His tag was expressed in HEK293 cells. Recombinant LDL receptor was further labled by Biotin for detection by avidin |
| Immunogen Sequence: |
MKPKMKYSTN KISTAKWKNT ASKALCFKLG KSQQKAKEVC PMYFMKLRSG LMIKKEACYF RRETTKRPSL KTGRKHKRHL VLAACQQQST VECFAFGISG VQKYTRALHD SSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCV |
| Post Translational Modifications | N- and O-glycosylated. Ubiquitinated by MYLIP leading to degradation. |
| Function | Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. |
| Protein Name | Low-Density Lipoprotein ReceptorLdl Receptor |
| Database Links | Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8964026Reactome: R-HSA-8964038Reactome: R-HSA-975634 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembraneClathrin-Coated PitGolgi ApparatusEarly EndosomeLate EndosomeLysosomeRapidly Endocytosed Upon Ligand Binding |
| Alternative Protein Names | Low-Density Lipoprotein Receptor proteinLdl Receptor proteinLDLR protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
