Human LCK protein (Recombinant) (N-His) (STJP010830)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human LCK-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | LCK |
| Gene ID | 3932 |
| Uniprot ID | LCK_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Arg242-Pro509 |
Additional Info
| Post Translational Modifications | Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling. Dephosphorylation at Tyr-394 by DUSP22 negatively regulates T-cell receptor signaling. Myristoylation is required prior to palmitoylation. Palmitoylation regulates association with the plasma membrane and could be mediated by ZDHHC2. 'Lys-63'-linked ubiquitinated at Lys-99 and Lys-276 by UBR2.this modification is required for autophosphorylation at Tyr-394. |
| Function | Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2. |
| Protein Name | Tyrosine-Protein Kinase LckLeukocyte C-Terminal Src KinaseLskLymphocyte Cell-Specific Protein-Tyrosine KinaseProtein Yt16Proto-Oncogene LckT Cell-Specific Protein-Tyrosine KinaseP56-Lck |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1257604Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-164944Reactome: R-HSA-167590Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-210990Reactome: R-HSA-2219530Reactome: R-HSA-2424491Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-389513Reactome: R-HSA-389948Reactome: R-HSA-6811558Reactome: R-HSA-9013407Reactome: R-HSA-9020558Reactome: R-HSA-9670439Reactome: R-HSA-9706374 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCytoplasmCytosolPresent In Lipid Rafts In An Inactive Form |
| Alternative Protein Names | Tyrosine-Protein Kinase Lck proteinLeukocyte C-Terminal Src Kinase proteinLsk proteinLymphocyte Cell-Specific Protein-Tyrosine Kinase proteinProtein Yt16 proteinProto-Oncogene Lck proteinT Cell-Specific Protein-Tyrosine Kinase proteinP56-Lck proteinLCK protein |
Information sourced from Uniprot.org