| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human LAG-3/CD223-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human LAG3 at 2 Mu g/mL (100 Mu L/well) can bind Anti-LAG3 antibody with a linear range of 13-40 ng/mL.2. Measured by its binding ability in a functional ELISA. Immobilize |
| Gene Symbol: |
LAG3 |
| Gene ID: |
3902 |
| Uniprot ID: |
LAG3_HUMAN |
| Immunogen: |
Recombinant Human LAG-3/CD223 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu23-Leu450) of human LAG-3/CD223 (Accession #NP_002277.4) fused with a 6×His tag at the C-terminus. |
| Tissue Specificity | Primarily expressed in activated T-cells and a subset of natural killer (NK) cells. |
| Post Translational Modifications | Lymphocyte activation gene 3 protein: Proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region, leading to release of Secreted lymphocyte activation gene 3 protein (sLAG-3). ADAM10 mediates constitutive cleavage, but cleavage increases following T-cell activation, whereas shedding by ADAM17 is induced by TCR signaling in a PRKCQ-dependent manner. |
| Function | Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as FGL1. FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function. Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1. Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells. Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function. Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation. Binds MHC class II (MHC-II).the precise role of MHC-II-binding is however unclear. Secreted lymphocyte activation gene 3 protein: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. |
| Protein Name | Lymphocyte Activation Gene 3 ProteinLag-3Cd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 ProteinSlag-3 |
| Database Links | Reactome: R-HSA-2132295 |
| Cellular Localisation | Lymphocyte Activation Gene 3 Protein: Cell MembraneSingle-Pass Type I Membrane ProteinSecreted Lymphocyte Activation Gene 3 Protein: SecretedProduced Following Cleavage Of The Main Chain |
| Alternative Protein Names | Lymphocyte Activation Gene 3 Protein proteinLag-3 proteinCd Antigen Cd223 Cleaved Into - Secreted Lymphocyte Activation Gene 3 Protein proteinSlag-3 proteinLAG3 proteinFDC protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
