Human Kallikrein-11/KLK11 protein (Recombinant) (C-His) (STJP002583)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Kallikrein-11/KLK11 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asn250) of human KLK11/Kallikrein-11 (Accession #NP_006844.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Kallikrein-11/KLK11-C-His protein was developed in hek293 cells using the region Met1-Asn250. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl) , in the presence of 5, 5 NA Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >141.40 pmol/min/Mu g. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | KLK11 |
| Gene ID | 11012 |
| Uniprot ID | KLK11_HUMAN |
| Immunogen | Recombinant Human Kallikrein-11/KLK11 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asn250) of human KLK11/Kallikrein-11 (Accession #NP_006844.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Met1-Asn250 |
| Immunogen Sequence | MRILQLILLALATGLVGGET RIIKGFECKPHSQPWQAALF EKTRLLCGATLIAPRWLLTA AHCLKPRYIVHLGQHNLQKE EGCEQTRTATESFPHPGFNN SLPNKDHRNDIMLVKMASPV SITWAVRPLTLSSRCVTAGT SCLISGWGSTSSPQLRLPHT LRCANITIIEHQKCENAYPG NITDTMVCASVQEGGKDSCQ GDSGGPLVCNQSLQGIISWG QDPCAITRKPGVYTKVCKY |
Additional Info
| Post Translational Modifications | About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen. |
| Function | Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. |
| Protein Name | Kallikrein-11Hk11HippostasinSerine Protease 20Trypsin-Like Protease Cleaved Into - Kallikrein-11 Inactive Chain 1 - Kallikrein-11 Inactive Chain 2 |
| Database Links | |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Golgi ApparatusSecreted |
| Alternative Protein Names | Kallikrein-11 proteinHk11 proteinHippostasin proteinSerine Protease 20 proteinTrypsin-Like Protease Cleaved Into - Kallikrein-11 Inactive Chain 1 - Kallikrein-11 Inactive Chain 2 proteinKLK11 proteinPRSS20 proteinTLSP proteinUNQ649 proteinPRO1279 protein |
Information sourced from Uniprot.org