| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >9209 pmol/min/Mu g. |
| Gene Symbol: | KLK1 |
| Gene ID: | 3816 |
| Uniprot ID: | KLK1_HUMAN |
| Immunogen: | Recombinant Human Kallikrein-1/KLK1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met 1-Ser 262) of human Kallikrein 1 (Accession #NP_002248) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | MWFLVLCLALSLGGTGAAPP IQSRIVGGWECEQHSQPWQA ALYHFSTFQCGGILVHRQWV LTAAHCISDNYQLWLGRHNL FDDENTAQFVHVSESFPHPG FNMSLLENHTRQADEDYSHD LMLLRLTEPADTITDAVKVV ELPTEEPEVGSTCLASGWGS IEPENFSFPDDLQCVDLKIL PNDECKKAHVQKVTDFMLCV GHLEGGKDTCVGDSGGPLMC DGVLQGVTSWGYVPCGTPN |
| Tissue Specificity | Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. |
| Post Translational Modifications | The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. |
| Function | Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva.Neisseria is an obligate commensal of the nasopharyngeal mucosa. |
| Protein Name | Kallikrein-1Kidney/Pancreas/Salivary Gland KallikreinTissue Kallikrein |
| Database Links | Reactome: R-HSA-381426 |
| Alternative Protein Names | Kallikrein-1 proteinKidney/Pancreas/Salivary Gland Kallikrein proteinTissue Kallikrein proteinKLK1 protein |
Information sourced from Uniprot.org