SDS-PAGE of recombinant human K-Ras protein under reducing conditions stained with Coomassie blue

Human K-Ras protein (Recombinant) (His-Tag) (STJP016749)

Name: Human K-Ras protein (Recombinant) (His-Tag) (STJP016749)
SKU: STJP016749
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

HostE.coli

ImmunogenHuman

STJP016749

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General Information

Short Description	Recombinant-Human K-Ras-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
Applications	SDS-PAGE
Host	E.coli
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration	0.5 mg/mL
Formulation	Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% Glycerol
Storage Instruction	For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene Symbol	KRAS
Gene ID	3845
Uniprot ID	RASK_HUMAN
Accession Number	AAH13572.1
Immunogen	Human
Immunogen Region	1-185aa
Immunogen Sequence	MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG HEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV REIRKHKEKM SKDGKKKKKK SKTKC

Additional Info

Post Translational Modifications	Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180. Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.
Protein Name	Gtpase Kras K-Ras 2 Ki-Ras C-K-Ras C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed
Database Links	Reactome: R-HSA-112412 Reactome: R-HSA-1169092 Reactome: R-HSA-1236382 Reactome: R-HSA-1250196 Reactome: R-HSA-1250347 Reactome: R-HSA-1433557 Reactome: R-HSA-167044 Reactome: R-HSA-171007 Reactome: R-HSA-179812 Reactome: R-HSA-180336 Reactome: R-HSA-186763 Reactome: R-HSA-1963640 Reactome: R-HSA-210993 Reactome: R-HSA-2179392 Reactome: R-HSA-2424491 Reactome: R-HSA-2428933 Reactome: R-HSA-2871796 Reactome: R-HSA-375165 Reactome: R-HSA-4086398 P01116-2 Reactome: R-HSA-442982 Reactome: R-HSA-5218921 Reactome: R-HSA-5621575 Reactome: R-HSA-5637810 Reactome: R-HSA-5654688 Reactome: R-HSA-5654693 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5658442 Reactome: R-HSA-5673000 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802953 Reactome: R-HSA-6802955 Reactome: R-HSA-74751 Reactome: R-HSA-8849471 Reactome: R-HSA-8851805 Reactome: R-HSA-8951936 Reactome: R-HSA-9026519 Reactome: R-HSA-9027284 Reactome: R-HSA-9028731 Reactome: R-HSA-9034864 Reactome: R-HSA-9607240 Reactome: R-HSA-9634285 Reactome: R-HSA-9634635 Reactome: R-HSA-9648002 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9674555 Reactome: R-HSA-9680350 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9753510 Reactome: R-HSA-9753512
Cellular Localisation	Cell Membrane Lipid-Anchor Cytoplasmic Side Endomembrane System Cytoplasm Cytosol Isoform 2b: Cell Membrane
Alternative Protein Names	Gtpase Kras protein K-Ras 2 protein Ki-Ras protein C-K-Ras protein C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed protein KRAS protein KRAS2 protein RASK2 protein

Information sourced from Uniprot.org

Citations

View product citations for antibody STJP016749 on CiteAb