Human ITK protein (Recombinant) (N-His) (STJP016067)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ITK-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | ITK |
| Gene ID | 3702 |
| Uniprot ID | ITK_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Glu173-Leu620 |
Additional Info
| Post Translational Modifications | Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain. Ubiquitinated. |
| Function | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3. |
| Protein Name | Tyrosine-Protein Kinase Itk/TskInterleukin-2-Inducible T-Cell KinaseIl-2-Inducible T-Cell KinaseKinase EmtT-Cell-Specific KinaseTyrosine-Protein Kinase Lyk |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-2871809 |
| Cellular Localisation | CytoplasmNucleusLocalizes In The Vicinity Of Cell Surface Receptors In The Plasma Membrane After Receptor Stimulation |
| Alternative Protein Names | Tyrosine-Protein Kinase Itk/Tsk proteinInterleukin-2-Inducible T-Cell Kinase proteinIl-2-Inducible T-Cell Kinase proteinKinase Emt proteinT-Cell-Specific Kinase proteinTyrosine-Protein Kinase Lyk proteinITK proteinEMT proteinLYK protein |
Information sourced from Uniprot.org