Human IRF8 protein (Recombinant) (N-His) (STJP005158)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP005158
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General Information

Short DescriptionRecombinant-Human IRF8-N-His protein was developed from e.coli for the region N-His. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolIRF8
Gene ID3394
Uniprot IDIRF8_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionGly128-Val426

Additional Info

Post Translational Modifications Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes. Ubiquitination leads to its degradation by the proteasome. Sumoylated with SUMO3. Desumoylated by SENP1.
Function Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor. Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection. Positively regulates macroautophagy in dendritic cells. Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1.
Protein Name Interferon Regulatory Factor 8
Irf-8
Interferon Consensus Sequence-Binding Protein
H-Icsbp
Icsbp
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-909733
Cellular Localisation Nucleus
Cytoplasm
In Resting Macrophages
Localizes In The Cytoplasm
Translocated In The Nucleus Upon Ifn-Gamma Induction
Alternative Protein Names Interferon Regulatory Factor 8 protein
Irf-8 protein
Interferon Consensus Sequence-Binding Protein protein
H-Icsbp protein
Icsbp protein
IRF8 protein
ICSBP1 protein

Information sourced from Uniprot.org

Citations

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