• The image depicts the log of the fold change of NK genes regulated due to co-culture with 3D7-infected erythrocytes (iRBCs) or with the cytokines IL-12 andIL-18.
  • NK92 cells were kept in two different environments for 24 hours prior to the co-culture: in normal cell medium NA >medium medium NA > (+rIL2; NK92 nm) and in cell medium NA >medium without rIL-2 (starvation medium NA >medium; NK92s). Cells from both environments were co-cultured with 3D7 schizont-infected erythrocytes and uRBCs (NK92-RBCs ratio: 1:3) in their respective growth medium. As a positive control, cells were also incubated with a mixture of IL-12 and IL-18 (and MBL, respectively; 100 ng/106 cells each). After the indicated time of incubation at 37°C and 5% CO2, NK cells from the co-culture as well as cells incubated without RBCs were stained for 30 min at 4°C with fluorochrome-conjugated antibodies for surface CD56 (APC) , CD3 (PE) , CD16 (FITC) , CD69 (FITC) , CD25 (PE) in parallel with the appropriate isotype controls. Cells were also internally stained with the IFN-Gamma (PE) antibody (all…
  • Purified human na i ve CD4 T cells were stimulated with irradiated (100Gy) allogeneic Epstein-Barr virus (EBV) – transformed B cells (1∶1 ratio) in complete medium NA >RPMI-8 medium at 105 cells/mL in round-bottom 96-well plate. Th1-biased cultures contained recombinant human IL-2 (5 ng/mL) , recombinant human IL-12 (20 ng/mL) and anti-IL-4 (5 µg/ml& ). Th2-biased cultures contained recombinant human IL-2 (5 ng/mL) , recombinant human IL-4 (20 ng/mL& ) , anti-IL-12 (5 µg/ml, ebioscience) and anti-IFN Gamma (5 µg/ml& ). Fresh medium containing 5 ng/mL IL-2 was added if necessary to cultures showing strong proliferation. The cultures were restimulated and expanded every seven days.
  • Co-culture supernatants from indicated groups were analyzed for IL-2, IL-12 by ELISA; mean±SD pg/ml shown.

Human Interleukin-12 protein (Recombinant) (STJP000505)

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STJP000505

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Interleukin-12-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution pH7.5.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: The ED (50) was determined by the dose-dependent proliferation ofactivated human T lymphoblasts. cells was found to be in the range of.1-0.2 ng/ml. NA
Gene Symbol: IL12B
Gene ID: 3593
Uniprot ID: IL12B_HUMAN
Immunogen Region: Mature chain
Immunogen: DNA sequence encoding Human Interleukin-12 mature chain was expressed in HEK cells. NA
Immunogen Sequence: MCHQQLVISW FSLVFLASPL VAIWELKKDV YVVELDWYPD APGEMVVLTC DTPEEDGITW TLDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSHS LLLLHKKEDG IWSTDILKDQ KEPKNKTFLR CEAKNYSGRF TCWWLTTIST DLTFSVKSSR GSSDPQGVTC GAATLSAERV RGDNKEYEYS VECQEDSACP AAEESLPIEV MVDAVHKLKY ENYTSSFF
Post Translational Modifications Known to be C-mannosylated in the recombinant protein.it is not yet known for sure if the wild-type protein is also modified.
Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Protein Name Interleukin-12 Subunit Beta
Il-12b
Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit
Clmf P40
Il-12 Subunit P40
Nk Cell Stimulatory Factor Chain 2
Nksf2
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9020591
Reactome: R-HSA-9020933
Cellular Localisation Secreted
Alternative Protein Names Interleukin-12 Subunit Beta protein
Il-12b protein
Cytotoxic Lymphocyte Maturation Factor 40 Kda Subunit protein
Clmf P40 protein
Il-12 Subunit P40 protein
Nk Cell Stimulatory Factor Chain 2 protein
Nksf2 protein
IL12B protein
NKSF2 protein

Information sourced from Uniprot.org

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