Human IL5 (Interleukin-5) Sandwich ELISA Kit (STJE0002047)

Name: Human IL5 (Interleukin-5) Sandwich ELISA Kit (STJE0002047)
SKU: STJE0002047
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002047

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

Short Description	This IL5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	IL5
Gene ID	3567
Uniprot ID	IL5_HUMAN
Specificity	This kit recognizes Human IL-5 in samples. No significant cross-reactivity or interference between Human IL-5 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively.
Protein Name	Interleukin-5 Il-5 B-Cell Differentiation Factor I Eosinophil Differentiation Factor T-Cell Replacing Factor Trf
Database Links	Reactome: R-HSA-512988 Reactome: R-HSA-5673001 Reactome: R-HSA-912526
Cellular Localisation	Secreted
Alternative ELISA Names	Interleukin-5 ELISA kit Il-5 ELISA kit B-Cell Differentiation Factor I ELISA kit Eosinophil Differentiation Factor ELISA kit T-Cell Replacing Factor ELISA kit Trf ELISA kit IL5 ELISA kit
Specificity	This kit recognizes Human IL-5 in samples. No significant cross-reactivity or interference between Human IL-5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	47.22	99.98	392.09	49.15	98.65	419.66
Standard deviation	2.54	5.06	21.17	3.15	4.92	13.51
CV (%)	5.38	5.06	5.40	6.41	4.99	3.22