• (a) Monocyte-derived LC were stimulated with either vehicle or PAF in the presence of neutralizing antibody to IL-15, IL-6R, and/or IL-23, or control Ig, and cocultured with antiCD3/CD28-activated CD4+ T cells for 5 days.
  • Frozen female CD34+ CBCs were supplied by Bio-Resource Center. C34+ CBCs were cultured in hematopoietic culture medium [serum-free X-Vivo10 containing 50 ng/mL IL-6, 50 ng/mL sIL-6, 50 ng/mL SCF, ten ng/mL TPO, and 20 ng/mL Flt3/4 ligand ]. Reprogrammed cells were cultured in feeder-less primate ES cell medium Repro FF (,. No. RCHEMD004) , ReproFF2 (ReproCELL, cat No. RCHEMD006) , mTeSR1 ( catalog number 05850) or E8 (16) supplemented with five ng/mL bFGF (total bFGF ten ng/mL) on Pronectin F-coated dishes. Passage of human iPSCs was previously described
  • HEK293T cells were transfected with mc_mock, mc_sTNFR-Fc, or mc_anti-IL-6R and the conditioned media were collected 24 h post-transfection. A-FLS were incubated with or without human IL-6 (100 ng/mL&, , ) , sIL-6 (100 ng/mLocky, ) , and TNF Alpha (20 ng/mL& ) for 72 h. uring the incubationA-FLS were treated with the conditioned media of HEK293T cells transfected with mc_mock, mc_sTNF-Fc, mc_anti-IL-6 or PBS (as a negative control). Cell proliferation was assessed using the cell counting kit-8 (CCK-8, , ) , according to the manufacturer NA s instructions.
  • To analyze the amounts of anti-IL-6R antibody and sTNFR2-Fc protein expressed by the transfected cells, the culture media of HEK293T cells transfected with the indicated minicircle vectors were analyzed by ELISA at 24 h post-transfection. The levels of sTNFR2-Fc were quantified using human sTNF-R (80 kDa) Platinum ELISA (eBioscience, San Diego, CA) , according to the manufacturer NA s instructions. ELISA was performed to determine the levels of anti-IL-6R antibodies. Briefly, a 96-well microtiter plate was coated with commercial anti-human IL-6R antibodies (B-R6; eBioscience) at a concentration of 0. 5  Mu g/mL in coating buffer, and incubated overnight at 4°C. The plate was washed five times with washing buffer, and incubated with 1  Mu g/mL of human sIL-6R at room temperature (RT) for 1 h. After washing, the plate was incubated with blocking solution for 1 h at RT, followed by incubation with 100  Mu L of serially diluted tocilizumab (used as a standard) , and the conditioned media from HEK293T cells were transfected…

Human IL-6 Receptor (IL-6R) protein (Recombinant) (STJP000392)

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STJP000392

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human IL-6 Receptor (IL-6R)-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: 0.2 Mu m filtered PBS solution, pH7.2.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: Recombinant IL-6R (CD126) activity was determined by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/ml. NA
Gene Symbol: IL6R
Gene ID: 3570
Uniprot ID: IL6RA_HUMAN
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of human IL-6 receptor (CD126) including a C-terminal PolyHis tag was expressed in HEK293 cells. NA
Tissue Specificity Isoform 2: Expressed in peripheral blood mononuclear cells and weakly found in urine and serum. 1%-20% of the total sIL6R in plasma is generated by alternative splicing.
Post Translational Modifications A short soluble form is released from the membrane by proteolysis. The sIL6R is formed mostly by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding, but is also directly secreted from the cells after alternative mRNA splicing. mIL6R is cleaved by the proteases ADAM10 and ADAM17. Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis.
Function Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). Isoform 1: Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Isoform 2: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. Soluble interleukin-6 receptor subunit alpha: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis.
Protein Name Interleukin-6 Receptor Subunit Alpha
Il-6 Receptor Subunit Alpha
Il-6r Subunit Alpha
Il-6r-Alpha
Il-6ra
Il-6r 1
Membrane Glycoprotein 80
Gp80
Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha
Sil6r
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-110056
Reactome: R-HSA-112411
Reactome: R-HSA-6785807
Reactome: R-HSA-9616222
Reactome: R-HSA-9679191
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Soluble Interleukin-6 Receptor Subunit Alpha: Secreted
Alternative Protein Names Interleukin-6 Receptor Subunit Alpha protein
Il-6 Receptor Subunit Alpha protein
Il-6r Subunit Alpha protein
Il-6r-Alpha protein
Il-6ra protein
Il-6r 1 protein
Membrane Glycoprotein 80 protein
Gp80 protein
Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha protein
Sil6r protein
IL6R protein

Information sourced from Uniprot.org

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