| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Recombinant-Human IL-5-N-His-Avi protein was developed from hek293 and has a target region of N-His-Avi. For use in research applications. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: | IL5 |
| Gene ID: | 3567 |
| Uniprot ID: | IL5_HUMAN |
| Immunogen Region: | Ile20-Ser134 |
| Immunogen: | DNA sequence encoding Human IL-5 including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Function | Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. |
| Protein Name | Interleukin-5 Il-5 B-Cell Differentiation Factor I Eosinophil Differentiation Factor T-Cell Replacing Factor Trf |
| Database Links | Reactome: R-HSA-512988 Reactome: R-HSA-5673001 Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-5 protein Il-5 protein B-Cell Differentiation Factor I protein Eosinophil Differentiation Factor protein T-Cell Replacing Factor protein Trf protein IL5 protein |
Information sourced from Uniprot.org
