Human IL-33 (Interleukin-33) Antibody Pair (STJAP00188)

SKU:
STJAP00188-50

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Host: Goat/Goat
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Short Description: This IL-33 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA.
Clonality: Polyclonal/Polyclonal
Conjugation: Unconjugated/Biotin
Formulation: Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Purification: Capture Purification: Antigen Affinity.
Detection Purification: Antigen Affinity.
Concentration: 0.5 mg/mL
Storage Instruction: Store at-20°C for long term, and avoid freeze-thaw cycles.
Gene Symbol: IL33
Gene ID: 90865
Uniprot ID: IL33_HUMAN
Immunogen: Capture immunogen: Recombinant Human IL-33 protein.
Detection Immunogen: Recombinant Human IL-33 protein.
Post Translational Modifications The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase.
Function Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation.
Protein Name Interleukin-33
Il-33
Interleukin-1 Family Member 11
Il-1f11
Nuclear Factor From High Endothelial Venules
Nf-Hev Cleaved Into - Interleukin-33
95-270 - Interleukin-33
99-270 - Interleukin-33
109-270
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-5689880
Reactome: R-HSA-6811558
Reactome: R-HSA-9014843
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Cytoplasmic Vesicle
Secretory Vesicle
Secreted
Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore Following Cleavage By Cela1
Associates With Heterochromatin And Mitotic Chromosomes
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative Antibody Pair Names Interleukin-33 antibody pair
Il-33 antibody pair
Interleukin-1 Family Member 11 antibody pair
Il-1f11 antibody pair
Nuclear Factor From High Endothelial Venules antibody pair
Nf-Hev Cleaved Into - Interleukin-33 antibody pair
95-270 - Interleukin-33 antibody pair
99-270 - Interleukin-33 antibody pair
109-270 antibody pair
IL33 antibody pair
C9orf26 antibody pair
IL1F11 antibody pair
NFHEV antibody pair

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance