• Biotinylated Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Biotinylated Human IL-3, His Tag at 5 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 1. 3 Mu g/ml determined by ELISA.

Human IL-3 protein (Recombinant) (N-His-Avi) {Biotin} (STJP001624)

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STJP001624

£597.50 - £1,839.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human IL-3-N-His-Avi protein was developed from hek293 and has a target region of N-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: IL3
Gene ID: 3562
Uniprot ID: IL3_HUMAN
Immunogen Region: Ala20-Phe152
Immunogen: DNA sequence encoding Human IL-3 including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Function Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK.
Protein Name Interleukin-3
Il-3
Hematopoietic Growth Factor
Mast Cell Growth Factor
Mcgf
Multipotential Colony-Stimulating Factor
P-Cell-Stimulating Factor
Database Links Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-8939247
Reactome: R-HSA-912526
Cellular Localisation Secreted
Alternative Protein Names Interleukin-3 protein
Il-3 protein
Hematopoietic Growth Factor protein
Mast Cell Growth Factor protein
Mcgf protein
Multipotential Colony-Stimulating Factor protein
P-Cell-Stimulating Factor protein
IL3 protein

Information sourced from Uniprot.org

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