Human IL-3 (IL-3) Antibody Pair (STJAP00724)
General Information
| Short Description | This IL-3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R07-4N1/R09-1S1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL3 |
| Gene ID | 3562 |
| Uniprot ID | IL3_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Activated T-cells, mast cells, natural killer cells. |
| Function | Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK. |
| Protein Name | Interleukin-3Il-3Hematopoietic Growth FactorMast Cell Growth FactorMcgfMultipotential Colony-Stimulating FactorP-Cell-Stimulating Factor |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-8939247Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-3 antibody pairIl-3 antibody pairHematopoietic Growth Factor antibody pairMast Cell Growth Factor antibody pairMcgf antibody pairMultipotential Colony-Stimulating Factor antibody pairP-Cell-Stimulating Factor antibody pairIL3 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-3 (IL-3) Antibody Pair (STJAP00724)
This antibody pair is designed to detect human Interleukin-3 (IL-3), corresponding to UniProt entry P08700. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. The capture clone is R07-4N1 and the detection clone is R09-1S1.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Working concentrations for both the capture and detection antibodies are to be determined by the end user. Titration experiments are recommended to establish optimal conditions for each specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, they should be aliquoted and kept at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Both clones in this pair were raised in rabbit against a recombinant human IL-3 protein. No additional information on the specific immunogen region is provided in the product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.