• On the left, histogram plots for 1 donor is shown and, on the right, IL-21R expression on day 4 is displayed for 5 donors.
  • MFBI Th1/Th2 Flow Cytomix Multiplex kits (eBioscience) were used to measure the concentration of cytokines in the serum two hours after s. c. treatment with soluble peptide, while IL-21 and IL-10 Flow Cytomix simplex kits (both eBioscience) were used to assay cell culture supernatant. Fluorescence intensity was measured on a FACS Calibur flow cytometer and data were analysed using FlowCytomix Pro software (eBioscience). Conventional sandwich ELISA were performed to quantify cytokine concentration in cell culture supernatant (harvested at 24 hours after re-stimulation for IL-2, at 72 hours for IFN-Gamma and IL-10) using matched antibody pairs (all ). IL-2 coating, JES6-1A12 (2 Mu g ml−1) , biotinylated, JES6-5H4 (0. 5 Mu g ml−1). IFN-Gamma : coating R4-6A2 (2 Mu g ml−1) , biotinylated, XMG1. 2 (0. 5 Mu g ml−1). IL-10: coating JES5-2A5 (2 Mu g ml−1) , biotinylated SXC-1 (0. 5 Mu g ml−1). Optical change was measured witha SpectraMax 190 microplate reader ; cytokine concentration was calculated using Microplate Manager software. Intracellular cytokine staining of splenocytes was performed after a 3 hour stimulation with…

Human IL-21R (CD360) protein (Recombinant) (STJP000690)

SKU:
STJP000690

£228.50 - £2,057.50
Shipping:
Free Shipping
Current Stock:
Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human IL-21R (CD360)-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised 0.2 Mu m filtered PBS solution, pH7.2.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: Measured by its ability to inhibit IL21 induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. NA
Gene Symbol: IL21R
Gene ID: 50615
Uniprot ID: IL21R_HUMAN
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of human IL-21R receptor including a C terminal His tag was expressed in HEK293 cells. NA
Tissue Specificity Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
Post Translational Modifications C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
Function This is a receptor for interleukin-21.
Protein Name Interleukin-21 Receptor
Il-21 Receptor
Il-21r
Novel Interleukin Receptor
Cd Antigen Cd360
Database Links Reactome: R-HSA-9020958
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names Interleukin-21 Receptor protein
Il-21 Receptor protein
Il-21r protein
Novel Interleukin Receptor protein
Cd Antigen Cd360 protein
IL21R protein
NILR protein
UNQ3121 protein
PRO10273 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance