| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | 1.Measured in a cell proliferation assay using HT-2 Mouse T cells. The ED50 for this effect is 0.25-0.5 ng/mL, corresponding to a specific activity of 2.0 × 106~4.0× 106 units/mg.|2.Measured by its binding ability in |
| Gene Symbol: | IL2 |
| Gene ID: | 3558 |
| Uniprot ID: | IL2_HUMAN |
| Immunogen Region: | Ala21-Thr153 |
| Immunogen: | Active Recombinant Human IL-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala21-Thr153) of human IL2 (Accession #NP_000577.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | APTSSSTKKTQLQLEHLLLD LQMILNGINNYKNPKLTRML TFKFYMPKKATELKHLQCLE EELKPLEEVLNLAQSKNFHL RPRDLISNINVIVLELKGSE TTFMCEYADETATIVEFLNR WITFCQSIISTLT |
| Function | Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells. |
| Protein Name | Interleukin-2 Il-2 T-Cell Growth Factor Tcgf Aldesleukin |
| Database Links | Reactome: R-HSA-5673001 Reactome: R-HSA-8877330 Reactome: R-HSA-9020558 Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-2 protein Il-2 protein T-Cell Growth Factor protein Tcgf protein Aldesleukin protein IL2 protein |
Information sourced from Uniprot.org