Human IL-18 (Interleukin-18) Antibody Pair (STJAP00038)
General Information
| Short Description | This IL-18 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | IL18 |
| Gene ID | 3606 |
| Uniprot ID | IL18_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human IL-18 protein.Detection Immunogen: Recombinant Human IL-18 protein. |
Additional Info
| Post Translational Modifications | The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18. |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18Il-18IboctadekinInterferon Gamma-Inducing FactorIfn-Gamma-Inducing FactorInterleukin-1 GammaIl-1 Gamma |
| Database Links | Reactome: R-HSA-448706Reactome: R-HSA-5620971Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9012546 Q14116-2Reactome: R-HSA-9660826 |
| Cellular Localisation | CytoplasmCytosolSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1Casp4 Or Casp5 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Pair Names | Interleukin-18 antibody pairIl-18 antibody pairIboctadekin antibody pairInterferon Gamma-Inducing Factor antibody pairIfn-Gamma-Inducing Factor antibody pairInterleukin-1 Gamma antibody pairIl-1 Gamma antibody pairIL18 antibody pairIGIF antibody pairIL1F4 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-18 (Interleukin-18) Antibody Pair (STJAP00038)
This antibody pair detects human Interleukin-18 (IL-18), corresponding to UniProt accession Q14116. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are rabbit polyclonal antibodies purified by antigen affinity chromatography and supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant human IL-18 protein. Both were subsequently purified by antigen affinity chromatography.
Both components should be stored at -20 degrees C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.