| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | 1.Recombinant Human IL1 Beta protein was measured by NF-Kappa B reporter assay in HEK293 cells. The ED50 for this effect is 1-10 pg/mL, corresponding to a specific activity of 1×108~1×109 units/mg.|2.Measured in a cel |
| Gene Symbol: | IL1B |
| Gene ID: | 3553 |
| Uniprot ID: | IL1B_HUMAN |
| Immunogen Region: | Ala117-Ser269 |
| Immunogen: | Recombinant Human IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6×His tag at the |
| Immunogen Sequence: | APVRSLNCTLRDSQQKSLVM SGPYELKALHLQGQDMEQQV VFSMSFVQGEESNDKIPVAL GLKEKNLYLSCVLKDDKPTL QLESVDPKNYPKKKMEKRFV FNKIEINNKLEFESAQFPNW YISTSQAENMPVFLGGTKGG QDITDFTMQFVSS |
| Tissue Specificity | Expressed in activated monocytes/macrophages (at protein level). |
| Post Translational Modifications | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. (Microbial infection) Cleavage by S.pyogenes cysteine protease SpeB promotes its activation independently of CASP1. |
| Function | Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection. |
| Protein Name | Interleukin-1 Beta Il-1 Beta Catabolin |
| Database Links | Reactome: R-HSA-448706 Reactome: R-HSA-5620971 Reactome: R-HSA-5660668 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-9020702 Reactome: R-HSA-9660826 |
| Cellular Localisation | Cytoplasm Cytosol Secreted Lysosome Extracellular Exosome The Precursor Is Cytosolic In Response To Inflammasome-Activating Signals Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome Cleaved And Secreted Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore In Contrast The Precursor Form Is Not Released Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Protein Names | Interleukin-1 Beta protein Il-1 Beta protein Catabolin protein IL1B protein IL1F2 protein |
Information sourced from Uniprot.org