Human IgG4 (IgG4) Antibody Pair (STJAP00689)
General Information
| Short Description | This IgG4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 3D8/9G12 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IGHG4Uniprot ID=IGHG4_HUMAN" |
| Immunogen | Recombinant human IgG4 |
Additional Info
| Post Translational Modifications | Glycosylation on Asn-177 is required for interaction with Fc receptors and ability to activate the complement pathway. (Microbial infection) Deglycosylation on Asn-177 by S.pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway. |
| Function | Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. |
| Protein Name | Immunoglobulin Heavy Constant Gamma 4Ig Gamma-4 Chain C Region |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cell MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Pair Names | Immunoglobulin Heavy Constant Gamma 4 antibody pairIg Gamma-4 Chain C Region antibody pairIGHG4 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IgG4 (IgG4) Antibody Pair (STJAP00689)
This antibody pair detects human IgG4, corresponding to UniProt entry P01861. Reactivity is confirmed for human samples only.
Both antibodies are mouse monoclonal antibodies (clones 3D8 and 9G12) supplied as affinity-purified liquids at 1 mg/mL. The capture antibody is formulated in PBS with 0.09% sodium azide, and the detection antibody is formulated in PBS with 0.01% thimerosal.
This pair has been validated for ELISA only. A dilution of 1:1000 is recommended for this application.
Both antibodies were raised against recombinant human IgG4. Both are mouse monoclonals of IgG1 isotype and are supplied unconjugated.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles.
No. This antibody pair is supplied for research use only and is not intended for diagnostic or therapeutic applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.