Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) Sandwich ELISA Kit (STJE0019763)

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STJE0019763
€204.38 - €446.88
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description : This IGFBP-7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IGFBP7
Gene ID: 3490
Uniprot ID: IBP7_HUMAN
Specificity: This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated.
Function Binds IGF1 and IGF2 with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Acts as a ligand for CD93 to play a role in angiogenesis.
Protein Name Insulin-Like Growth Factor-Binding Protein 7
Ibp-7
Igf-Binding Protein 7
Igfbp-7
Igfbp-Rp1
Mac25 Protein
Pgi2-Stimulating Factor
Prostacyclin-Stimulating Factor
Tumor-Derived Adhesion Factor
Taf
Database Links Reactome: R-HSA-2559582
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative ELISA Names Insulin-Like Growth Factor-Binding Protein 7 ELISA kit
Ibp-7 ELISA kit
Igf-Binding Protein 7 ELISA kit
Igfbp-7 ELISA kit
Igfbp-Rp1 ELISA kit
Mac25 Protein ELISA kit
Pgi2-Stimulating Factor ELISA kit
Prostacyclin-Stimulating Factor ELISA kit
Tumor-Derived Adhesion Factor ELISA kit
Taf ELISA kit
IGFBP7 ELISA kit
MAC25 ELISA kit
PSF ELISA kit
Specificity This kit recognizes Human IGFBP-7 in samples. No significant cross-reactivity or interference between Human IGFBP-7 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-96 91
EDTA plasma (n=8) 96-107 101
Cell culture media (n=8) 90-103 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 91.33 264.92 952.33 91.69 263.98 1011.09
Standard deviation 6.17 13.27 45.14 6.32 10.59 36.20
CV (%) 6.76 5.01 4.74 6.89 4.01 3.58

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